The first set of Round of 16 midweek matches began on Tuesday, 27 August with three fixtures live and exclusive on 10 Play.
Our feature clash took us to the home of Lions FC while elsewhere we had an all Victorian clash at Hume City while we headed west for the final encounter.
Australia Cup: Important Links
Lions FC vs Western Sydney Wanderers
The Match of the Day took us to Luxury Paints Stadium in Queensland with The Lions welcoming Western Sydney Wanderers.
It was the Wanderers' second visit to the Sunshine state after they saw off Brisbane City in the Round of 32.
A dominant display from Alen Stajcic's side saw them run away with the win as they booked their ticket to Adelaide for the Quarter Finals.
- Lions FC vs Western Sydney Wanderers: Full Match Replay
- Lions FC vs Western Sydney Wanderers: Highlights
Hume City FC vs FC Melbourne Srbija
Two NPL clubs went head to head at Hume City Stadium in the second of the three fixtures.
One goal separated the two sides on this occasion with Aamir Abdallah's second half goal proving the difference as Hume put an end to Melbourne Srbija's fairytale Cup run.
- Hume City FC vs FC Melbourne Srbija: Full Match Replay
- Hume City FC vs FC Melbourne Srbija: Highlights
Olympic Kingsway SC vs Adelaide United
The Tuesday night action wrapped up in the west as Olympic Kingsway met Adelaide United.
Adelaide survived a late scare from the hosts as they ran out 3-2 winners thanks to goals from Austin Ayoub, Callum O'Connell and Luka Jovanovic.
Special mention goes to Kingsway's Liam Boland who overtook Besart Berisha to become the outright top goal scorer in Australia Cup history.