Senator Lambie faced a tight race to retain her Senate seat against One Nation's Lee Hanson, whose mother Pauline founded the party.
On Tuesday, the Australian Electoral Commission declared Senator Lambie had triumphed, scraping into Tasmania's fifth upper chamber spot as Liberal Richard Colbeck took the sixth seat and left Ms Hanson out in the cold.
Senator Lambie thanked her supporters and vowed to help those doing it tough over the next six years.
"Representing Tasmania and bringing the voices and concerns of the people to Canberra is what gets me out of bed in the morning!" she said in a statement.