The toll of the current outbreak now stands at 114.
There was also one new case acquired from overseas and 79,200 tests processed on Tuesday.
The health department on Wednesday confirmed there are now 19,861 active infections across the state.
More than 38,000 Victorians received a COVID vaccine on Tuesday at a state-run hub.
Visitors to the Royal Children's Hospital will now have to undergo rapid antigen testing after a potentially infectious parent visited the newborn intensive care unit.
Hospital chief executive Bernadette McDonald told reporters a father visited the unit on Thursday and Friday last week and returned a positive result on Monday, notifying the hospital that evening.
Of the 29 babies in the unit at the time, two are tier one close contacts and the remainder are tier two, with the infants and their families all now isolating. No transmission to the babies has yet been found.
"It is a very challenging circumstances to find the balance between allowing mums and dads to come and see their children and spend time with their children ... and trying to manage to keep everyone - families, staff, patients - safe from COVID-19," Ms McDonald said.
Ms McDonald said while the numbers varied, on average there were about five patients at the children's hospital with COVID-19 at any one time.
They are cared for in negative pressure rooms and the hospital currently has enough of those rooms to meet demand.
Opposition health spokeswoman Georgie Crozier said the scare could have been avoided entirely if rapid antigen testing was already in place.
"It's again a failure of the Andrews government to prepare our hospitals," she told reporters at parliament.
The hospital's cancer ward was exposed to the virus at the beginning of this month, after a patient's parent tested positive for COVID-19 after visiting over at least four days.
Elective surgeries will be paused from Thursday as the state prepares for an influx of coronavirus patients in coming weeks. Currently there are 675 people in care, with 144 in intensive care units and 100 on ventilators.
Victoria is also seeking to recruit up to 1000 healthcare workers from outside Australia to help the system cope as virus patients grow.
As of Tuesday, almost 60 per cent of Victorians aged over 16 were fully vaccinated, while 86.7 per cent have received one COVID-19 vaccine dose.
The state government has also been drafting specific pandemic laws to replace the current state of emergency powers, which expire on December 15.
Reason Party MP Fiona Patten told reporters at parliament she is hopeful the laws will give Health Minister Martin Foley the power to sign off public health orders rather than the chief health officer, like in NSW.
"Any legislation needs to be accountable, it needs to be transparent, and I think we're hearing murmurings that will be the case," Ms Patten said.
Following concerns poppies will not be able to be sold ahead of Remembrance Day on November 11, Veterans Minister Shaun Lean has confirmed the annual fundraiser will be able to go ahead.