Qantas and Jetstar pilots, cabin crew and airport workers will have until November 15 to be fully jabbed.
The deadline for other employees to be fully jabbed is March 31, 2022.
Qantas boss Alan Joyce on Wednesday said having a fully vaccinated workforce would help stop the spread of the virus.
"One crew member can fly into multiple cities and come into contact with thousands of people in a single day," he said.
"Making sure they are vaccinated given the potential of this virus to spread is so important and I think it's the kind of safety leadership people would expect from us."