The Australian Technical Advisory Group on Immunisation has recommended a second booster be administered for Australians over 65, Indigenous Australians over 50, those in disability care and those who are immunocompromised over the age of 16.
The doses will be rolled out to those groups from April 4 at pharmacies, GPs and vaccine clinics.
The ATAGI advice recommends the second booster be given to those groups between four and six months after the first booster.
The advice was given ahead of a winter which is expected to bring with it a spike in COVID-19 cases alongside a rise in flu cases.
Health Minister Greg Hunt also announced the government's biosecurity emergency determination will not be renewed after April 17, when the determination expires.
Mr Hunt said the move would mean there would be no need for pre-flight testing for arrivals into Australia.
The health minister will also lose certain powers.
However, arrivals to Australia will still need to be vaccinated and wear masks on flights.
Mr Hunt announced the flu vaccine program will also be rolled out on April 4.
LATEST 24-HOUR COVID-19 DATA FROM ACROSS AUSTRALIA:
NSW: 23,702 cases, seven deaths, 1182 in hospital including 43 in ICU
Victoria: 9244 cases, nine deaths, 253 in hospital including 19 in ICU