The Ye Olde Fighting Cocks, in St Albans, England, claims to be the country's oldest pub after opening its doors in 793.
But two years of the Covid pandemic have meant the business could no longer meet its financial obligations.
In a post on its Facebook's page, manager Christo Tofalli explained the pub was already facing financial issues before the pandemic, but extended closures have sealed the pub's fate.
"Along with my team, I have tried everything to keep the pub going," he said.
"However, the past two years have been unprecedented for the hospitality industry and have defeated all of us who have been trying our hardest to ensure this multi-award-winning pub could continue trading into the future."
Tofalli went on to say that he is "heartbroken" at the decision to close its doors.
"This pub has been so much more than just a business to me, and I feel honoured to have played even a small part in its history," he said.
The pub building is owned by Mitchells & Butlers, and the company told CNN there is the potential for the pub to reopen.
"We are currently exploring all opportunities for the site's future and hope to reopen the pub under new management as soon as possible," a spokesperson said.